Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: Cell viability ( A ), LDH release ( B ), and caspase-1 activity ( C ) of the A549 cells treated with phages vB_SauM-A, vB_SauM-C, vB_SauM-D, 1.0 mg/mL lactoferrin, 2.0 mg/mL linezolid, and bacteriophages supplemented with 1 mg/mL lactoferrin in comparison to the untreated negative control and positive control. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using one-way ANOVA, with *** p < 0.001

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Activity Assay, Comparison, Negative Control, Positive Control

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: The CFU/mL of MRSA strains 70 ( A ), 110 ( B ), and 203 ( C ) in infected A549 cell line after treatment with phages vB_SauM-A, vB_SauM-C, and vB_SauM-D, lactoferrin, linezolid, or phage + Lf combination after 3 h and 6 h post-treatment. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using the Kruskal–Wallis test followed by Dunn’s multiple comparison test for values with nonparametric distribution, with *** p < 0.001

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Infection, Comparison

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: Cell viability ( A , B , C ) and LDH release ( D , E , F ) from the infected A549 cells treated with phages vB_SauM-A, vB_SauM-C, vB_SauM-D, 1.0 mg/mL lactoferrin, 2.0 mg/mL linezolid, and phage + Lf combination in comparison to the untreated negative control and positive control. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using one-way ANOVA, with *** p < 0.001, ** p < 0.01, and * p < 0.05

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Infection, Comparison, Negative Control, Positive Control

Journal: Applied Microbiology and Biotechnology

Article Title: Addition of lactoferrin increases efficacy of three Kayviruses and limits the inflammatory response in pulmonary epithelial cells

doi: 10.1007/s00253-025-13695-9

Figure Lengend Snippet: Inflammasome activation measured by caspase-1 activity in the infected A549 cells treated with phages vB_SauM-A, vB_SauM-C, vB_SauM-D, 1.0 mg/mL lactoferrin, 2.0 mg/mL linezolid, and phage + Lf combination in comparison to the untreated negative control and positive control. Arithmetic mean of triplicates with error bars representing SD. Statistical analysis was performed using the Kruskal–Wallis test followed by Dunn’s multiple comparison test for values with nonparametric distribution, with *** p < 0.001

Article Snippet: Human carcinoma lung epithelial cell line A549 (ATCC, CCL-185) (ATCC, Manassas, VA, USA) were cultured in 75-cm 2 Culture Flask (Corning, New York, NY, USA) in a F-12 K culture medium (ATCC, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS; ATCC, Manassas, VA, USA), at 37 °C in a humidified atmosphere of 95% air and 5% CO 2 in the HeraCell 150 incubator (Heraeus, Hanau, Germany) in accordance with the supplier’s guidelines.

Techniques: Activation Assay, Activity Assay, Infection, Comparison, Negative Control, Positive Control